Gruppo Pinzani
Coordinator of Research Team
Name: Pamela Pinzani
Position: Associate Professor in Clinical Biochemistry;
Name of Research Team Laboratory: Clinical Biochemistry and Clinical Molecular Biology
e-mail:pamela.pinzani@unifi.it
telephone number:+39 055 2758411
Brief biographical sketch of the Coordinator (date of birth, degrees and Universities where they have been obtained)
Date of birth: 30/05/1947, Florence
Biologist, Ph.D. of the University of Florence, Full Professor of Clinical Biochemistry and Clinical Molecular Biology, Faculty of Medicine, University of Florence.
Member of the scientific board of the Doctorate/PhD Program in
Scienze Biomediche
Member of the following Scientific Societies
Research Team
1) Pamela Pinzani Associate Professor in Clinical Biochemistry and Clinical Molecular Biology, 15/11/1961
2) Stefania Gelmini PhD,24/05/1965
3) Lisa Simi PhD, 18/09/1972
4) Francesca Salvianti PhD, 30/07/1977
5) Irene Mancini PhD, 21/06/1984
6) Filomena Costanza, PhD Student in Biomedical Sciences 12/08/1988
Current research interests
Personalized Medicine
Liquid Biopsy: Circulating Tumor Cell (CTC) and circulating DNA/RNA isolation and characterization Pharmacogenomics
Tumor DNA genotyping
DNA methylation
Gene expression analysis
Standardization: implementation of CEN and ISO technical standards on pre-analytical phase for molecular studies
Current sources of funding
1) Horizon2020: SPIDIA4P-SPIDIA for Personalized Medicine – Standardisation of generic Pre-analytical procedures for Invitro Diagnostics-G.A. n. 733112
2) Ministero della Salute/Regione Toscana: Circulating cell-free biomarkers in the diagnosis and follow-up of differentiated thyroid cancer- Project Code RF-2011-02352294
3) Regione Toscana: FAS-OMITERC-Applicazione degli OMIcs dalla biopsia solida alla biopsia liquida per una TERapia personalizzata del Cancro- CUPD76D16000180002
10 best publications of the last 5 years
Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer.
De Luca F, Rotunno G, Salvianti F, Galardi F, Pestrin M, Gabellini S, Simi L, Mancini I, Vannucchi AM, Pazzagli M, Di Leo A, Pinzani P
ONCOTARGET 2016 Mar;
Prevalence and number of circulating tumour cells and microemboli at diagnosis of advanced NSCLC.
Mascalchi M, Falchini M, Maddau C, Salvianti F, Nistri M, Bertelli E, Sali L, Zuccherelli S, Vella A, Matucci M, Voltolini L, Pegna AL,
Luconi M, Pinzani P, Pazzagli M
J CANCER RES CLIN 2016 Jan; 142: 195-200
Single circulating tumor cell sequencing as an advanced tool in cancer management.
Salvianti F, Pazzagli M, Pinzani P
EXPERT REV MOL DIAGN 2016; 16: 51-63
Feasibility of a workflow for the molecular characterization of single cells by next generation sequencing.
Salvianti F, Rotunno G, Galardi F, De Luca F, Pestrin M, Vannucchi AM, Di Leo A, Pazzagli M, Pinzani P
Biomol Detect Quantif 2015 Sep; 5: 23-9
Heterogeneity of PIK3CA mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients.
Pestrin M, Salvianti F, Galardi F, De Luca F, Turner N, Malorni L, Pazzagli M, Di Leo A, Pinzani P
MOL ONCOL 2015 Apr; 9: 749-57
Tumor-Related Methylated Cell-Free DNA and Circulating Tumor Cells in Melanoma.
Salvianti F, Orlando C, Massi D, De Giorgi V, Grazzini M, Pazzagli M, Pinzani P
Front Mol Biosci 2015; 2: 76
Circulating cell-free DNA in cancer.
Pinzani P, Salvianti F, Orlando C, Pazzagli M
Methods Mol Biol 2014; 1160: 133-45
Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, Günther K, Verderio P, Pizzamiglio S, Ciniselli CM, Zhang H, Korenková V, Rainen L, Bar T, Kubista M, Gelmini S.
PLoS One. 2014 Nov 10;9(11):e112293. doi: 10.1371/journal.pone.0112293. eCollection 2014
Multiparametric analysis of cell-free DNA in melanoma patients.
Salvianti F, Pinzani P, Verderio P, Ciniselli CM, Massi D, De Giorgi V, Grazzini M, Pazzagli M, Orlando C.
PLoS One. 2012;7(11):e49843. doi: 10.1371/journal.pone.0049843. Epub 2012 Nov 27.
Pazzagli M, Malentacchi F, Simi L, Orlando C, Wyrich R, Günther K, Hartmann CC, Verderio P, Pizzamiglio S, Ciniselli CM, Tichopad A, Kubista M, Gelmini S.
Methods. 2013 Jan;59(1):20-31. doi: 10.1016/j.ymeth.2012.10.007. Epub 2012 Oct 27.
Previous research experiences
Chemiluminescence and Bioluminescence
Immunoassay and luminescence assay in Endocrinology
Antioxidant capacity
Quantitative PCR (qPCR) assay
Main scientific contributions
- M.P is an international recognized expert in Clinical Biochemistry, member of several
societies of Laboratory Medicine: SiBioC (Società Italiana di Biochimica Clinica), IFCC (International
Federation of Clinical Chemistry and Laboratory Medicine), EFLM (European Federation of Laboratory
Medicine). He participated to three European collaborative projects: 2001-2002 Coordinator of the Project
FP5 EU "La Vinci-Linnaeus-Da Darwin Initiative"; 2004-2007 Coordinator of the Project FP6 EU "Multi-
National External Quality Assay (EQA) Programmes in Clinical Molecular Diagnostics based on Performance
and Interpretation of PCR assay methods including dissemination and training”; 2008-2013 Member of the
Project FP7 EU “SPIDIA: Standardisation and improvement of generic pre-analytical tools and procedures
for in vitro diagnostics”. Today, he is the Project Leader of the Reference ISO/NP 20091 “Molecular in vitro
diagnostic examinations -- Specifications for pre-examination processes for blood -- Circulating cell free
DNA” and Reference ISO/NP 20185 “Molecular in-vitro diagnostic examinations -- Specifications for preexamination processes for blood -- Genomic DNA”.
Collaborations
Université Laval, Canada
Prof Massimo Mannelli (Dip. SBSC)
Prof.ssa Michaela Luconi (Dip. SBSC)
Prof. Mario Mascalchi (Dip SBSC)
Prof Enrico Mini (Dip Medicina Sperimentle e Clinica)
Prof.ssa Daniela Massi (Dip Chirurgia e Medicina Translazionale)
Prof Angelo di Leo, Nuovo Ospedali S Stefano Prato
Dr.ssa Cinzia Mazzini AOU Careggi-SOD Oculistica
Prof Nicola Pimpinelli (Dip Chirurgia e Medicina Translazionale)
Prof Francesco Lolli (Dip. SBSC)
Ultimo aggiornamento
27.05.2021